Cell cycle (Georgetown, Tex.) 2007,
PMID: 17314511
Koch, Heike B; Zhang, Ru; Verdoodt, Berlinda; Bailey, Aaron; Zhang, Chang-Dong; Yates, John R; Menssen, Antje; Hermeking, Heiko
The c-MYC oncogene encodes a transcription factor, which is sufficient and necessary for the induction of cellular proliferation. However, the c-MYC protein is a relatively weak transactivator suggesting that it may have other functions. To identify protein interactors which may reveal new functions or represent regulators of c-MYC we systematically identified proteins associated with c-MYC in vivo using a proteomic approach. We combined tandem affinity purification (TAP) with the mass spectral multidimensional protein identification technology (MudPIT). Thereby, 221 c-MYC-associated proteins were identified. Among them were 17 previously known c-MYC-interactors. Selected new c-MYC-associated proteins (DBC-1, FBX29, KU70, MCM7, Mi2-beta/CHD4, RNA Pol II, RFC2, RFC3, SV40 Large T Antigen, TCP1alpha, U5-116kD, ZNF281) were confirmed independently. For association with MCM7, SV40 Large T Antigen and DBC-1 the functionally important MYC-box II region was required, whereas FBX29 and Mi2-beta interacted via MYC-box II and the BR-HLH-LZ motif. In addition, regulators of c-MYC activity were identified: ectopic expression of FBX29, an E3 ubiquitin ligase, decreased c-MYC protein levels and inhibited c-MYC transactivation, whereas knock-down of FBX29 elevated the concentration of c-MYC. Furthermore, sucrose gradient analysis demonstrated that c-MYC is present in numerous complexes with varying size and composition, which may accommodate the large number of new c-MYC-associated proteins identified here and mediate the diverse functions of c-MYC. Our results suggest that c-MYC, besides acting as a mitogenic transcription factor, regulates cellular proliferation by direct association with protein complexes involved in multiple synthetic processes required for cell division, as for example DNA-replication/repair and RNA-processing. Furthermore, this first comprehensive description of the c-MYC-associated sub-proteome will facilitate further studies aimed to elucidate the biology of c-MYC.
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Manually curated Databases
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IRef Biogrid Interaction:
MYC
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MARS
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IRef Biogrid Interaction:
MYC
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XPO5
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IRef Biogrid Interaction:
MYC
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NOP56
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IRef Biogrid Interaction:
MYC
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TRRAP
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IRef Biogrid Interaction:
MYC
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MAX
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IRef Biogrid Interaction:
MYC
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MAX
(direct interaction, fluorescent resonance energy transfer)
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IRef Biogrid Interaction:
CCAR2
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MYC
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IRef Biogrid Interaction:
CCAR2
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MYC
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IRef Biogrid Interaction:
IQGAP2
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MYC
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IRef Biogrid Interaction:
POLR2B
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MYC
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IRef Biogrid Interaction:
MYC
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GTF2I
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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MMS19
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IRef Biogrid Interaction:
FBXW8
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
FBXW8
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MYC
(direct interaction, two hybrid)
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IRef Biogrid Interaction:
MYBBP1A
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RAF1
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MYC
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IRef Biogrid Interaction:
GTF3C3
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MYC
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IRef Biogrid Interaction:
GTF3C1
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MYC
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IRef Biogrid Interaction:
BCR
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HUWE1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MSH2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HDAC1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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POLR2A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
NCL
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RUVBL1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMA2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HSPH1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IKBKAP
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SLC25A1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMB4
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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RNGTT
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
GTF3C4
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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RPP30
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ZNF281
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MYC
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IRef Biogrid Interaction:
ZNF281
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MYC
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IRef Biogrid Interaction:
PSMC3
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MYC
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IRef Biogrid Interaction:
MYC
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HIST1H2BL
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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NDUFA5
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCT4
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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IGF2BP2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MRPS34
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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RPN1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ALDH18A1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PSMD2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
AKAP8
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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OAT
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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HNRNPF
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PLOD3
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
UBE3C
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMD3
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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NUP153
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SLIT2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ERCC3
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DNAJA2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CEPT1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCT8
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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TUBGCP2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMA5
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KPNA4
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMC6
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ARL1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TOP2A
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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DHX15
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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MOGS
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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DPM1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ARF4
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CSE1L
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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XPO1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PSMA4
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
AASS
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
XPOT
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DIMT1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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NDUFS1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MSH3
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SUCLG1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SLC25A13
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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YWHAB
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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ASS1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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GSG2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMA7
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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LONP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IPO7
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SHOC2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KPNB1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TUBG1
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MYC
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IRef Biogrid Interaction:
WDR77
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MYC
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IRef Biogrid Interaction:
RANBP2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HNRNPK
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MYC
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IRef Biogrid Interaction:
PELO
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MYC
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IRef Biogrid Interaction:
MYC
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HSPB1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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MYO5C
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
C1QBP
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
XRCC5
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IRS4
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
NDUFS3
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DDX52
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
FBL
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KPNA2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IDH3B
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
NDUFA8
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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MYO9A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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YWHAE
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SMARCA5
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MYC
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IRef Biogrid Interaction:
MYC
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CCT5
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IRef Biogrid Interaction:
PTGES2
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MYC
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IRef Biogrid Interaction:
MYC
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HSPD1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HNRNPA0
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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SQSTM1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MRPS22
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MYC
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IRef Biogrid Interaction:
PSMB1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
AKAP8L
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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NEK9
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HADHB
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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EIF3E
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CHTF18
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YWHAQ
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PTPN14
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IRef Biogrid Interaction:
PSMB3
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MYC
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IRef Biogrid Interaction:
PLK1
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MYC
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MYC
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SKP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ABI3BP
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MYC
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IRef Biogrid Interaction:
MYC
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CYR61
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RNH1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ACOT8
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SQRDL
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MYC
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IRef Biogrid Interaction:
MYC
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DDX1
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IRef Biogrid Interaction:
SARS
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MYC
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IRef Biogrid Interaction:
POLD1
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MYC
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GTF3C2
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MYC
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MYC
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ATP5C1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RFC3
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MYC
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IRef Biogrid Interaction:
XRCC6
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MYC
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IRef Biogrid Interaction:
ILVBL
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MYC
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IRef Biogrid Interaction:
IDH3A
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MYC
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IRef Biogrid Interaction:
COPG2
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MYC
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IRef Biogrid Interaction:
MYC
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PSMA1
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IRef Biogrid Interaction:
MYC
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HIGD1A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PSME4
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IRef Biogrid Interaction:
EIF3I
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MYC
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IRef Biogrid Interaction:
EEF1G
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
U2SURP
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SLC25A12
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PCBP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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DYNC1H1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
LMNA
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IPO13
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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RFC5
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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MAPKAP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
XRN1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
EIF4A1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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GANAB
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IRef Biogrid Interaction:
MYC
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TTC27
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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IRAK1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ETFA
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MYC
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IRef Biogrid Interaction:
GCDH
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IDH3G
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
FANCD2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PPT1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
EIF4ENIF1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PSMB5
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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TRMT1L
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PPP6C
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RPN2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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TCP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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CCT6A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PPP1CA
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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IGF2BP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PRKDC
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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UBR2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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NONO
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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IQGAP3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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CCT3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SPTLC1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMC2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCT2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PSMA3
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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PSMC4
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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SSR4
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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DNAJB6
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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SLC25A11
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ALDH1B1
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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P4HA1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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TMPO
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
NDUFS2
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MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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MYO9B
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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ATP5J2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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RFC2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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DNAJA3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
GCN1L1
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SNRNP200
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
NUP133
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
FBXW7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
POLE
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
TOP1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CHD4
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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SMC2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SMC4
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
SMARCC1
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TUBA4A
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
EFTUD2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
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ARHGEF2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
PRPF6
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DDX20
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
IPO4
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
LRPPRC
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TRIM28
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HDAC2
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HELLS
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
RUVBL2
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ZNF121
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MSH6
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
MCM7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
MCM7
(direct interaction, two hybrid)
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IRef Biogrid Interaction:
MYC
—
IPO9
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
COPG1
—
MYC
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MYC
—
ACTL6A
(physical association, affinity chromatography technology)
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IRef Intact Interaction:
MYC
—
CCAR2
(physical association, anti tag coimmunoprecipitation)
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IRef Intact Interaction:
MYC
—
CCAR2
(physical association, anti bait coimmunoprecipitation)
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IRef Intact Interaction:
MYC
—
CCAR2
(physical association, two hybrid)
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IRef Intact Interaction:
MYC
—
FBXW8
(physical association, anti tag coimmunoprecipitation)
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IRef Intact Interaction:
MYC
—
FBXW8
(physical association, two hybrid)
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IRef Intact Interaction:
Complex of MYC-MCM7-CCAR2-EFTUD2-XRCC6
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of 222 proteins
(association, tandem affinity purification)
-
IRef Intact Interaction:
MYC
—
CHD4
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of 13 proteins
(association, tandem affinity purification)
-
IRef Intact Interaction:
EFTUD2
—
MYC
(physical association, cosedimentation through density gradient)
-
IRef Intact Interaction:
MYC
—
ZNF121
(physical association, two hybrid)
-
IRef Intact Interaction:
Complex of 13 proteins
(physical association, cosedimentation through density gradient)
-
IRef Intact Interaction:
MYC
—
MCM7
(physical association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
MYC
—
MCM7
(physical association, anti bait coimmunoprecipitation)
-
IRef Intact Interaction:
MYC
—
MCM7
(physical association, two hybrid)
In total, 24091 gene pairs are associated to this article in curated databases